Project information
Regulation and manipulation of plant meiosis
- Project Identification
- CZ.02.1.01/0.0/0.0/15_003/0000479 (kod CEP: EF15_003/0000479)
- Project Period
- 1/2017 - 10/2022
- Investor / Pogramme / Project type
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Ministry of Education, Youth and Sports of the CR
- Operational Programme Research, Development and Education
- Priority axis 1: Strengthening capacities for high-quality research
- MU Faculty or unit
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Central European Institute of Technology
- Mgr. Karel Říha, Ph.D.
- doc. MVDr. Martin Anger, CSc.
- Albert Cairo Calzada, Ph.D.
- Mgr. Jaroslav Fulneček, CSc.
- Mgr. Anna Vargová, PhD.
Meiosis lies at the heart of sexual reproduction, the most successful reproductive strategy creating new genetic combinations every generation. This project aims at understanding the mechanisms that define meiotic division and gamete differentiation in plants; part of the effort will be establishing technologies allowing in vitro differentiation of male gametes, live imaging of meiosis and the manipulation of meiotic processes. The approaches will include microfluidics for in vitro cultivation of reproductive tissues coupled with time-laps microscopy (anther on chip technology), next generation sequencing and bioinformatics for identification of new meiotic regulators, CRISPR/Cas9 genome editing and development of techniques for the delivery of biologically active compounds into meiotic. This project well complements ongoing research activities at CEITEC and will strengthen its excellence at multiple levels: 1) It brings unique technology and instrumentation, which will yield important and highly visible discoveries. 2) It combines different expertise and contributes to the multidisciplinarity of the research centre. 3) It has applied potential in plant breeding. 4) It will facilitate establishing close ties to the Vienna Biocenter and promote cross-border collaboration with this prestigious research cluster.
Sustainable Development Goals
Masaryk University is committed to the UN Sustainable Development Goals, which aim to improve the conditions and quality of life on our planet by 2030.
Publications
Total number of publications: 17
2023
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A complex role of Arabidopsis CDKD;3 in meiotic progression and cytokinesis
Plant Direct, year: 2023, volume: 7, edition: 3, DOI
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High temperature increases centromere-mediated genome elimination frequency and enhances haploid induction in Arabidopsis
Plant Communications, year: 2023, volume: 4, edition: 3, DOI
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The biological functions of nonsense-mediated mRNA decay in plants: RNA quality control and beyond
Biochemical Society Transactions, year: 2023, volume: 51, edition: 1, DOI
2022
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Application of Chemical Inhibitors in Live Cell Imaging of Plant Meiosis Using Light Sheet Fluorescence Microscopy
Plant Gametogenesis. Methods in Molecular Biology, vol 2484, year: 2022, number of pages: 13 s.
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B1-type cyclins control microtubule organization during cell division in Arabidopsis
EMBO reports, year: 2022, volume: 23, edition: 1, DOI
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Meiotic exit in Arabidopsis is driven by P-body-mediated inhibition of translation
Science, year: 2022, volume: 377, edition: 6606, DOI
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Transcriptional and post-transcriptional regulation of young genes in plants
BMC Biology, year: 2022, volume: 20, edition: 1, DOI
2021
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A CENH3 mutation promotes meiotic exit and restores fertility in SMG7-deficient Arabidopsis
PLoS Genetics, year: 2021, volume: 17, edition: 9, DOI
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A hypomorphic allele of telomerase uncovers the minimal functional length of telomeres in Arabidopsis
Genetics, year: 2021, volume: 219, edition: 2, DOI
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High-Throughput Protein-Nucleic Acid Interaction Assay Based on Protein-Induced Fluorescence Enhancement
RNA REMODELING PROTEINS, year: 2021, number of pages: 9 s.