Small non-coding RNAs (ncRNAs) represent a significant, ubiquitous and ever-growing class of regulatory RNAs that are principally involved in control of many cellular processes of most organisms, regardless if bacterial or eukaryotic. Mechanisms of their function are not yet comprehensively understood. However, it is now clear that they are also significant clinically. The course will focus on methods enabling characterization of cellular content of all classes of small ncRNAs as well as providing insights into their function. In particular, we will teach methods allowing investigation of these molecules in all organisms, not only in human or mouse. The practical part will include ncRNA detection, data analysis, their integration as well as correlation of data obtained by various methods. During the course we will pay particular attention to following aspects: a) explanation and practicing of different approaches used for
ncRNA detection & characterization of their abundance;
ABSTRACT:
Principal themes and objectives of the event
b) detailed troubleshooting for all steps required to characterize ncRNA content and profiles;
c) comparison of analyses strategies and evaluation of obtained data;
d) experimental approaches for the identification of ncRNA 'targets' and for the functional
validation of their activity.